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著者: Vasanthapuram Ravi, Jaimie S Robinson, Brandy J Russell, Anita Desai, Nalini Ramamurty, David Featherstone, Barbara W Johnson
雑誌名: Am J Trop Med Hyg. 2009 Dec;81(6):1144-50. doi: 10.4269/ajtmh.2009.09-0144.
Abstract/Text
Infection with Japanese encephalitis virus (JEV) is a major public health problem in Asia. Detection of JEV-specific IgM in serum and cerebrospinal fluid (CSF) by the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) is currently the most widely used diagnostic method to detect JEV infection. Because of the possible presence of IgM cross-reactivity with other flaviviruses in serum and the high ratio of inapparent-to-apparent JEV infections, a positive result in serum only suggests a recent infection and not necessarily an encephalitic illness caused by JEV. Consequently, detection of JEV-specific IgM in CSF assumes great diagnostic relevance. We evaluated two commercial JEV MAC-ELISA kits using 60 CSF samples obtained from patients with acute encephalitis syndrome. The Panbio and XCyton kits had sensitivities of 65-80% and 95% and specificities of 90% and 97.5%, respectively. Performance information on these commercial JEV MAC-ELISA kits for CSF should assist in laboratory-based JE surveillance programs.
PMID 19996450 Am J Trop Med Hyg. 2009 Dec;81(6):1144-50. doi: 10.4269/ajtmh.2009.09-0144.
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